Turn on the spectrometer and let it warm up for at least 10 minutes. Change the chamber light to the desired wavelength on the spectrometer. Prepare a “blank.” Fill the cuvette halfway with the reaction solution that does not contain the unknown sample. Wipe off the sides of the cuvette with a Kim-wipe.Click to see full answer. Likewise, people ask, why do you need to calibrate a spectrometer?Spectrophotometer needs to be calibrated against a blank solution so that measurements after it can use the blank solution’s absorbance as a zero reference. A measure of the capacity of a substance to absorb light of a specified wavelength. what does it mean to calibrate something? : to adjust or mark (something, such as a measuring device) so that it can be used in an accurate and exact way. : to measure (something) in an exact and precise way. See the full definition for calibrate in the English Language Learners Dictionary. calibrate. transitive verb. People also ask, how often should a spectrophotometer be calibrated? once a year Why is it important to calibrate a colorimeter?Like with any piece of equipment or machinery, color measurement instruments need maintenance to assure that they continue to work correctly and with a predictably high degree of accuracy. Calibration allows us to set a baseline for the instrument and make sure that the baseline is maintained over time.